Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Dihydrostreptomycin sulfate (Dihydrostreptomycin sesquisulfate) 是一种氨基糖苷类抗生素,可研究牛、猪和羊的细菌性疾病。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
500 mg | ¥ 423 | 现货 | ||
1 g | ¥ 498 | 现货 |
产品描述 | Dihydrostreptomycin sulfate (Dihydrostreptomycin sesquisulfate) inhibits protein synthesis by binding to the 30S ribosomal subunit. Dihydrostreptomycin Sulfate is a semi-synthetic aminoglycoside antibiotic with bactericidal properties. This antibiotic is active against most gram-positive and gram-negative organisms and is used in the treatment of tuberculosis and tularemia. |
体外活性 | The primary mechanism of action of the antibiotic dihydrostreptomycin is binding to and modifying the function of the bacterial ribosome, thus leading to decreased and aberrant translation of proteins. It enters bacterial cells by binding to a specific site on MscL(mechanosensitive channel of large conductance) and modifying its conformation, thus allowing the passage of K+ and glutamate out of, and dihydrostreptomycin into, the cell[1]. |
体内活性 | Dihydrostreptomycin sulfate 会导致小鼠模型耳蜗损伤,而在Dihydrostreptomycin sulfate 诱发损伤后,螺旋韧带中的细胞可能会发生增殖。这一过程可能与耳蜗功能的恢复有关[2]。 |
激酶实验 | Briefly, the peptide substrate N-acetyl-Asp-Glu-Val-Asp-ρ-nitroanilide (Ac-DEVD-ρNA) is added to the cell lysates in assay buffer (50 mM HEPES, pH 7.4, 100 mM NaCl, 0.1% CHAPS, 10 mM dithiothreitol, 1 mM EDTA, 10% glycerol) and incubated at 37°C. The cleavage of the substrate is monitored at 405 nm. |
别名 | Dihydrostreptomycin sesquisulfate, 双氢链霉索 |
分子量 | 730.71 |
分子式 | C21H41N7O12·1·5H2SO4 |
CAS No. | 5490-27-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 10 mM
DMSO: Insoluble
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O | 1 mM | 1.3685 mL | 6.8427 mL | 13.6853 mL | 34.2133 mL |
5 mM | 0.2737 mL | 1.3685 mL | 2.7371 mL | 6.8427 mL | |
10 mM | 0.1369 mL | 0.6843 mL | 1.3685 mL | 3.4213 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Dihydrostreptomycin sulfate 5490-27-7 Microbiology/Virology Antibacterial Antibiotic inhibit Dihydrostreptomycin Sulfate Dihydrostreptomycin sesquisulfate Inhibitor 双氢链霉索 Dihydrostreptomycin Bacterial inhibitor